2-D Proteome Analysis Protocols by Andrew J. Link

Examining and contrasting the global protein of cell or tissue is the main purpose of this book. Their interaction with foregn agents is also discussed and will be of use for molecular or clinical laboratories. A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions.
`Author(s) :` Andrew J. Link	`Format :` Hardback Book
`ISBN-10 :` 0896035247	`ISBN-13 :` 9780896035249
`RRP :` £76.00	`Best available price :` £ / $
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Product Details:

Series Title : Methods in Molecular Biology

Country Publication : United States

Publication Date : 01/09/1998

Publisher : Humana Press Inc.,U.S.

Page Length : 450mm

Page Size : 152mm