Single Nucleotide Polymorphisms by Pui-Yan Kwok

Methods and Protocols

This volume presents essential tried and tested methods used in identifying new SNPs and in scoring down SNPs in many individuals. "Single Nucleotide Polymorphisms" provides up-to-date protocols that should enable all interested in genetic analysis to apply these approaches in their own studies. Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
`Author(s) :` Pui-Yan Kwok	`Format :` Hardback Book
`ISBN-10 :` 0896039684	`ISBN-13 :` 9780896039681
`RRP :` £61.00	`Best available price :` £50.36 / $
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Product Details:

Series Title : Methods in Molecular Biology

Country Publication : United States

Publication Date : 01/10/2002

Publisher : Humana Press Inc.,U.S.

Page Length : 275mm

Page Size : 229mm